Determining the prevalence of Escherichia coli, Salmonella, and shiga toxin-producing Escherichia coli in manure of dairy lagoons.

AIM: The aim of this study was to determine the prevalence of microbial pathogens in manure of dairy lagoons in California. METHODS AND RESULTS: To determine pathogens in dairy manure stored in anaerobic lagoons of dairy farm, an extensive field study was conducted across California to sample manure from 20 dairy farms. Samples were analyzed to determine the prevalence of indicator E. coli, Shiga toxin producing E. coli (STEC), Salmonella, and E. coli O157:H7. To test the E. coli, STEC, and Salmonella, we used agar culture-based method followed by polymerase chain reaction (PCR) method. In addition, a real- time PCR based method was used to determine the presence of E coli O157:H7. Study demonstrated that the prevalence of Salmonella in manure sample is lower than E. coli. The presence of Salmonella was found in 2.26% of the samples, and both the culture-based and PCR methods yielded comparable outcomes in detecting Salmonella. Moreover, approximately 11.30% of the total samples out of the 177 were identified as positive for STEC by qPCR. CONCLUSION: These findings demonstrate that indicator E. coli are abundantly present in anaerobic lagoons. However, the presence of STEC, and Salmonella is substantially low.

Variability of soft-bottom macrobenthic invertebrates at different spatial scales: Comparisons between habitats and seasons.

Studies focusing on patterns of spatial variation in marine soft-bottom assemblages suggest that variability is mainly concentrated at small spatial scale (from tens of centimeters to few meters), but there is still a lack of knowledge about the consistency of this spatial pattern across habitats and seasons. To address this issue, we quantified the variability in the structure of macrozoobenthic assemblages and in the abundance of dominant macroinvertebrate species in the Mellah Lagoon (Algeria) at three spatial scales, i.e., Plot (meters apart), Station (10's m apart) and Site (kms apart) scale, in Ruppia maritima (Ruppia) beds and unvegetated sediments (Unvegetated), and in two dates in winter and two dates in summer 2016. Spatial variability of the most dominant bivalve Mytilaster marioni varied significantly between habitats, but consistent across the two seasons, with a more heterogeneous distribution in Ruppia than in Unvegetated at the Station scale. Furthermore, a second-order interaction among the hierarchical nature of spatial variability, season and habitat emerged for the assemblage structure. Spatial variability between habitats varied significantly in winter, with the largest variation at the Plot scale in Unvegetated and more heterogenous assemblages at the Plot and Site scales than at the Station scale in Ruppia, but did not vary in summer when most of the variance was at the Site scale. We demonstrate that the scales of influence of the processes operating in the Mellah Lagoon are contingent on the specific habitat and/or period of the year at which the study was conducted, highlighting the importance of examining all these sources of variation simultaneously to increase the accuracy of explanatory models derived from the observed patterns in sedimentary environments.

Influence of trophic overlaps and trophic niche amplitude on microplastic intake of fish species in shallow areas of a neotropical coastal lagoon.

Plastic pollution is a global challenge that affects all marine ecosystems, and reflects all types of uses and activities of human society in these environments. In marine ecosystems, microplastics and mesoplastics interact with invertebrates and become available to higher predators, such as fish, which can ingest these contaminants. This study aimed to analyze how ecological food interactions (diet overlap and trophic niche amplitude) among fish species contribute to the ingestion of plastic particles. The gastrointestinal contents of six fish species (Atherinella brasiliensis, Eucinostomus melanopterus, Eucinostomus argenteus, Genidens genidens, Coptodon rendalli, and Geophagus brasiliensis) were analyzed to identify prey items and plastic ingestion. Based on the ontogenetic classification, A. brasiliensis, E. melanopterus, and G. genidens were divided into juveniles and adults, and the six fish species analyzed were divided into nine predator groups. Most of the plastics ingested by the fish species were blue microplastic (MP) fibers (< 0.05 mm) classified as polyester terephthalate, polyethylene, and polybutadiene. Considering all the analyzed predators, the average number and weight of plastics ingested per individual were 2.01 and 0.0005 g, respectively. We observed that predators with a high trophic overlap could present a relationship with the intake of MP fibers owing to predation on the same resources. In addition, we observed the general pattern that when a species expands its trophic diversity and niche, it can become more susceptible to plastic ingestion. For example, the species with the highest Levin niche amplitude, E. argenteus juveniles, had the highest mean number (2.9) of ingested MP fibers. Understanding the feeding ecology and interactions among species, considering how each predator uses habitats and food resources, can provide a better understanding of how plastic particle contamination occurs and which habitats are contaminated with these polluting substances.

Assessing the impact of an integrated educational program on Greek students' knowledge about coastal lagoons and attitudes towards marine environment conservation.

The primary objective of this study is to contribute to the conservation and sustainable use of seas by promoting Ocean Literacy. It investigates the impact of an educational program on Greek primary and secondary public school students' knowledge about coastal lagoons and attitudes towards marine environment conservation. An educational resource titled "Exploring the Coastal Lagoons" was developed to facilitate the non-formal educational intervention. The program involved classroom, fieldwork/outdoor and laboratory activities, focusing on enhancing understanding of coastal lagoons' abiotic and biotic characteristics and human interconnection. Results showed improved knowledge and slightly more positive attitudes after the didactic intervention. The study underlines the effectiveness of targeted educational interventions in marine sciences, suggesting that non-formal educational settings influence student outcomes more than family or informal sources. Younger students appeared more adaptable and responsive to educational stimuli. The study advocates for refined educational strategies integrating cognitive and emotional elements, emphasizing real nature experience.

Microbial diversity in dairy manure environment under liquid-solid separation systems.

In dairy manure, a wide array of microorganisms, including many pathogens, survive and grow under suitable conditions. This microbial community offers a tremendous opportunity for studying animal health, the transport of microbes into the soil, air, and water, and consequential impacts on public health. The aim of this study was to assess the impacts of manure management practices on the microbial community of manure. The key novelty of this work is to identify the impacts of various stages of manure management on microbes living in dairy manure. In general, the majority of dairy farms in California use a flush system to manage dairy manure, which involves liquid-solid separations. To separate liquid and solid in manure, Multi-stage Alternate Dairy Effluent Management Systems (ADEMS) that use mechanical separation systems (MSS) or weeping wall separation systems (WWSS) are used. Thus, this study was conducted to understand how these manure management systems affect the microbial community. We studied the microbial communities in the WWSS and MSS separation systems, as well as in the four stages of the ADEMS. The 16S rRNA gene from the extracted genomic DNA of dairy manure was amplified using the NovoSeq Illumina next-generation sequencing platform. The sequencing data were used to perform the analysis of similarity (ANOSIM) and multi-response permutation procedure (MRRP) statistical tests, and the results showed that microbial communities among WWSS and MSS were significantly different (p < 0.05). These findings have significant practical implications for the design and implementation of manure management practices in dairy farms.

Why coastal lagoons are so productive? Physical bases of fishing productivity in coastal lagoons.

Coastal lagoons are among the most productive marine ecosystems in the world. Annual primary production varies from 50 to > 500 g C m-2 year-1, being of the same order of magnitude as that of the upwelling areas. Many lagoons lie within the range of eutrophic (300-500 g C m-2 year-1) or hypereutrophic (> 500 g C m-2 year-1) conditions. The high productivity of coastal lagoons makes them subject of exploitation by many marine fishes and invertebrates, that use them as nursery areas and feeding grounds during their early life cycle phases, and most lagoons support important fisheries or maintain aquaculture exploitations. The high levels of their biological production can be explained by some of their common features as shallowness and the strong influence of terrestrial systems. Shallowness favors that the photic zone extends to the lagoon bottom and that wind can promote the resuspension of nutrients and organisms. The interaction with land also introduces significant amounts of nutrients. However, trophic variables can explain < 43 % of the fishing yields, and further than the trophic status of the lagoons, several works showed that the biological productivity of coastal lagoons can be explained by their geomorphological features such as the positive influence of shoreline development and the negative influence of depth. Using the Mar Menor lagoon as a case study, we propose that although nutrient inputs and light can be limiting factors for photosynthetic based productivity, increasing fishing yield up to a certain limit, the productivity of lagoons is mainly promoted by more general forces associated to physical and chemical gradients.

Spatio-temporal structuring and assembly of abundant and rare bacteria in the benthic compartment of a marginally eutrophic lagoon.

The investigations on ecological processes that structure abundant and rare sub-communities are limited from the benthic compartments of tropical brackish lagoons. We examined the spatial and temporal patterns in benthic bacterial communities of a brackish lagoon; Chilika. Abundant and rare bacteria showed differences in niche specialization but exhibited similar distance-decay patterns. Abundant bacteria were mostly habitat generalists due to their broader niche breadth, environmental response thresholds, and greater functional redundancy. In contrast, rare bacteria were mostly habitat specialists due to their narrow niche breadth, lower environmental response thresholds, and functional redundancy. The spatial patterns in abundant bacteria were largely shaped by stochastic processes (88.7 %, mostly dispersal limitation). In contrast, rare bacteria were mostly structured by deterministic processes (56.4 %, mostly heterogeneous selection). These findings provided a quantitative assessment of the different forces namely spatial, environmental, and biotic that together structured bacterial communities in the benthic compartment of a marginally eutrophic lagoon.

Unregulated Active and Closed Textile Mills Represent a Significant Vector of PFAS Contamination into Coastal Rivers.

Despite concerns over the ubiquity of per- and polyfluoroalkyl substances (PFAS), little is known about the diversity of input sources to surface waters and their seasonal dynamics. Frequent use of PFAS in textiles means both active and closed textile mills require evaluation as PFAS sources. We deployed passive samplers at seven sites in an urban river and estuary adjacent to textile mills in Southern Rhode Island (USA) over 12 months. We estimated monthly mass flows (g month-1) of perfluorohexanoic acid (PFHxA: 45±56), and perfluorooctanoic acid (PFOA: 30±45) from the upstream river influenced by an active mill. Average mass flows were 73-155% higher downstream, where historical textile waste lagoons contributed long chain perfluoroalkyl acids (PFAA). Mass flows of PFNA increased from 7.5 to 21 g month-1 between the upstream and downstream portions of the rivers. Distinct grouping of the two main PFAS sources, active textile mills and historical waste lagoons, were identified using principal components analysis. Neither suspect screening nor extractable organofluorine analysis revealed measurable PFAS were missing beyond the targeted compounds. This research demonstrates that both closed and active textile mills are important ongoing PFAS sources to freshwater and marine regions and should be further evaluated as a source category.

Spatial and Temporal Analysis of Water Quality in High Andean Lakes with Sentinel-2 Satellite Automatic Water Products.

The water of high Andean lakes is strongly affected by anthropic activities. However, due to its complexity this ecosystem is poorly researched. This study analyzes water quality using Sentinel-2 (S2) images in high Andean lakes with apparent different eutrophication states. Spatial and temporal patterns are assessed for biophysical water variables from automatic products as obtained from versions of C2RCC (Case 2 Regional Coast Color) processor (i.e., C2RCC, C2X, and C2X-COMPLEX) to observe water characteristics and eutrophication states in detail. These results were validated using in situ water sampling. C2X-COMPLEX appeared to be an appropriate option to study bodies of water with a complex dynamic of water composition. C2RCC was adequate for lakes with high transparency, typical for lakes of highlands with excellent water quality. The Yambo lake, with chlorophyll-a concentration (CHL) values of 79.6 ± 5 mg/m3, was in the eutrophic to hyper-eutrophic state. The Colta lake, with variable values of CHL, was between the oligotrophic to mesotrophic state, and the Atillo lakes, with values of 0.16 ± 0.1 mg/m3, were oligotrophic and even ultra-oligotrophic, which remained stable in the last few years. Automatic S2 water products give information about water quality, which in turn makes it possible to analyze its causes.

Sanctuaries are not inviolable: Haplosporidium pinnae as responsible for the collapse of the Pinna nobilis population in Lake Faro (central Mediterranean).

The rapid spread of the protozoan Haplosporidium pinnae is having a strong negative effect on Pinna nobilis populations. A case study on a residual population in Lake Faro (Sicily, Central Mediterranean), whose long-term monitoring has revealed a dramatic decline following the 2018-2020 mass mortality event, is presented. In the framework of such monitoring, we performed tissue sampling on nine living P. nobilis, detecting the pathogen in seven of them. In contrast, other pathogens associated with P. nobilis disease in other areas, i.e., Mycobacterium spp. and Vibrio mediterranei, were not recorded. The surviving individuals (approximately twenty) showed that brackish areas only weakly mitigate the effects of H. pinnae disease and might not be resolutive. Nevertheless, the results show that Lake Faro may constitute one of the last Mediterranean P. nobilis sanctuaries.

Measuring Marine Biotoxins in a Hypersaline Coastal Lagoon.

Marine biotoxins have posed a persistent problem along various coasts for many years. Coastal lagoons are ecosystems prone to phytoplankton blooms when altered by eutrophication. The Mar Menor is the largest hypersaline coastal lagoon in Europe. Sixteen marine toxins, including lipophilic toxins, yessotoxins, and domoic acid (DA), in seawater samples from the Mar Menor coastal lagoon were measured in one year. Only DA was detected in the range of 44.9-173.8 ng L-1. Environmental stressors and mechanisms controlling the presence of DA in the lagoon are discussed. As an enrichment and clean-up method, we employed solid phase extraction to filter and acidify 75 mL of the sample, followed by pre-concentration through a C18 SPE cartridge. The analytes were recovered in aqueous solutions and directly injected into the liquid chromatography system (LC-MS), which was equipped with a C18 column. The system operated in gradient mode, and we used tandem mass spectrometry (MS/MS) with a triple quadrupole (QqQ) in the multiple reaction monitoring mode (MRM) for analysis. The absence of matrix effects was checked and the limits of detection for most toxins were low, ranging from 0.05 to 91.2 ng L-1, depending on the compound. To validate the measurements, we performed recovery studies, falling in the range of 74-122%, with an intraday precision below 14.9% RSD.

Identification and quantification of Acanthamoeba spp. within seawater at four coastal lagoons on the east coast of Australia.

Acanthamoeba is an opportunistic free-living heterotrophic protist that is the most predominant amoeba in diverse ecological habitats. Acanthamoeba causes amoebic keratitis (AK), a painful and potentially blinding corneal infection. Major risk factors for AK have been linked to non-optimal contact lens hygiene practices and Acanthamoeba contamination of domestic and recreational water. This study investigated the incidence and seasonal variation of Acanthamoeba spp. within coastal lagoons located on the eastern coast of Australia and then examined the association between Acanthamoeba and water abiotic factors and bacterial species within the water. Water samples were collected from four intermittently closed and open lagoons (ICOLLs) (Wamberal, Terrigal, Avoca and Cockrone) every month between August 2019 to July 2020 except March and April. qPCR was used to target the Acanthamoeba 18S rRNA gene, validated by Sanger sequencing. Water abiotic factors were measured in situ using a multiprobe metre and 16S rRNA sequencing (V3-V4) was performed to characterise bacterial community composition. Network analysis was used to gauge putative associations between Acanthamoeba incidence and bacterial amplicon sequence variants (ASVs). Among 206 water samples analysed, 79 (38.3%) were Acanthamoeba positive and Acanthamoeba level was significantly higher in summer compared with winter, spring, or autumn (p = 0.008). More than 50% (23/45) water samples of Terrigal were positive for Acanthamoeba which is a highly urbanised area with extensive recreational activities while about 32% (16/49) samples were positive from Cockrone that is the least impacted lagoon by urban development. All sequenced strains belonged to the pathogenic genotype T4 clade except two which were of genotype clades T2 and T5. Water turbidity, temperature, intl1 gene concentration, and dissolved O2 were significantly associated with Acanthamoeba incidence (p < 0.05). The ASVs level of cyanobacteria, Pseudomonas spp., Candidatus spp., and marine bacteria of the Actinobacteria phylum and Acanthamoeba 18S rRNA genes were positively correlated (Pearson's r ≥ 0.14). The presence of Acanthamoeba spp. in all lagoons, except Wamberal, was associated with significant differences in the composition of bacterial communities (beta diversity). The results of this study suggest that coastal lagoons, particularly those in urbanised regions with extensive water recreational activities, may pose an elevated risk to human health due to the relatively high incidence of pathogenic Acanthamoeba in the summer. These findings underscore the importance of educating the public about the rare yet devastating impact of AK on vision and quality of life, highlighting the need for collaborative efforts between public health officials and educators to promote awareness and preventive measures, especially focusing lagoons residents and travellers.

Bathymetric data for Israeli coastal micro-estuaries.

Data from a bathymetric mapping project conducted in seven Israeli coastal micro-estuaries (Lachish, Sorek, Yarkon, Alexander, Hadera, Taninim, and Kishon) is presented. The data were collected by rowing a kayak along an S-shaped track through the estuaries. An echosounder equipped with a Global Positioning System (GPS) unit were mounted on the kayak. The data preparation consisted of a) manual removal of outliers, mostly caused by instrument echo in water depths below the instrument's 0.5 m minimum; b) correction of the measured water level to sea level; and c) interpolation of the sampling points into a regular grid using a terrain-following interpolation algorithm. For each of the estuaries, the raw measurements as a text (csv) file and the interpolated data both as a text (CSV) file and a GeoTiff file were produced.

Protist taxonomic and functional diversity in aquatic ecosystems of the Brazilian Atlantic Forest.

The Brazilian Atlantic Forest and its associated ecosystems are highly biodiverse but still understudied, especially with respect to eukaryotic microbes. Protists represent the largest proportion of eukaryotic diversity and play important roles in nutrient cycling and maintenance of the ecosystems in which they occur. However, much of protist diversity remains unknown, particularly in the Neotropics. Understanding the taxonomic and functional diversity of these organisms is urgently needed, not only to fill this gap in our knowledge, but also to enable the development of public policies for biological conservation. This is the first study to investigate the taxonomic and trophic diversity of the major protist groups in freshwater systems and brackish coastal lagoons located in fragments of the Brazilian Atlantic Forest by DNA metabarcoding, using high-throughput sequencing of the gene coding for the V4 region of the 18S rRNA gene. We compared α and ß diversity for all protist communities and assessed the relative abundance of phototrophic, consumer, and parasitic taxa. We found that the protist communities of coastal lagoons are as diverse as the freshwater systems studied in terms of α diversity, although differed significantly in terms of taxonomic composition. Our results still showed a notable functional homogeneity between the trophic groups in freshwater environments. Beta diversity was higher among freshwater samples, suggesting a greater level of heterogeneity within this group of samples concerning the composition and abundance of OTUs.Ciliophora was the most represented group in freshwater, while Diatomea dominated diversity in coastal lagoons.

Dissolved organic matter distribution in the water column and sediment pore water in a highly anthropized coastal lagoon (Mar Menor, Spain): Characteristics, sources, and benthic fluxes.

Coastal lagoons are among the most productive and biodiverse systems in the world and are important sentinels of climate change. The Mar Menor is one of the largest coastal lagoons in the Mediterranean, providing a variety of ecosystem services and resources to the community. However, in recent decades this lagoon has suffered drastic changes and degradation caused by human activities. We analyzed the concentration of dissolved organic carbon (DOC) and the optical properties of dissolved organic matter (DOM) in the water column and sediment pore water during the summer and winter of 2018 and during eighteen months from 2016 to 2018. Overall, we found that the composition of DOM is mainly related to and enhanced by anthropogenic activities and microbial metabolism. DOM enters the lagoon via urban and agricultural runoff, drainage systems, and wastewater treatment plants. Additionally, strong microbial metabolism in sediments leads to differences in DOM composition between water and sediments. In the water column, humic-like components accounted for 71 % of the total DOM, while protein-like compounds were most abundant in sediment pore water. We observed a strong seasonal variability associated with precipitation and the system collapse in 2016 (phytoplankton bloom), which resulted in the death of 80 % of macrophytes. The sediments act as a source of DOM to the overlying water, likely due to relatively high organic matter content and intense microbial activity, primarily through anaerobic pathways. Benthic fluxes of DOC ranged from 5.24 to 33.30 mmol m-2 d-1, being higher in winter than summer 2018 and decreasing from north to south, likely related to lower residence time in the northern basin, groundwater discharge and accumulation of organic matter from the dead meadows. We estimate a net flux of DOC from the Mar Menor toward the Mediterranean Sea of 1.57 × 107 mol yr-1.

Fate of microbial contamination in a South European Coastal Lagoon (Ria Formosa) under the influence of treated effluents dispersal.

AIM: Assessment of the fate of microbial contamination driven from treated wastewater disposal at a highly productive zone on a South European coastal lagoon (Ria Formosa). METHODS AND RESULTS: Microbial indicators of contamination (Total coliforms, Escherichia coli, and Enterococci) were evaluated monthly during September 2018-September 2020 at three study areas (Faro, Olhão, and Tavira) under different wastewater discharge flows and hydrodynamic conditions. Additional data on E. coli monitoring in bivalves, available from the national institution responsible for their surveillance was also considered. The maximum microbial contamination was found at Faro, the highest-load and less-flushed study area, contrasting the lowest contamination at Olhão, a lower-load and strongly flushed area. The wastewater impact decreased along the spatial dispersal gradients and during high water, particularly at Faro and Tavira study areas, due to a considerable dilution effect. Microbial contamination at Olhão increased during the summer, while at the other study areas seasonal evidence was not clear. Data also indicate that E. coli in bivalves from bivalve production zones next to the three study areas reflected the differentiated impact of the wastewater treatment plants effluents on the water quality of those areas. CONCLUSIONS: Effluent loads together with local hydrodynamics, water temperature, solar radiation, precipitation, and land runoff as well as seabirds populations and environmentally adapted faecal or renaturelized bacterial communities, contributed to microbial contamination of the study areas.

Estimating migration speed of glass eels during their colonization of a Mediterranean lagoon.

Migration speed can have important evolutionary consequences as it can affect the timing of arrival, remaining energy reserves, and habitat choice. Environmental conditions and individual phenotypic traits can impact the migration speed of individuals. In this way, estimating migration speed is of particular importance, especially for species under strong management strategies and colonizing highly diversified habitats, as is the case for the European eel. However, estimating the migration speed of glass eels, which is the life stage when eels colonize continental habitats, presents challenges due to typically low re-capture probabilities and difficulties in tagging individuals. Using recruitment time series at two sites, one at the sea connection and another inland, we estimated the temporal lag between the two migration peaks to compute migration speed. Because we worked on the Mediterranean coasts and in a lagoon, the weak tidal amplitudes may inhibit individuals from efficiently performing the selective tidal stream transport. We obtained migration speed values coherent with the few values available in the literature for Atlantic estuaries. The values we obtained that are lower than those obtained for Atlantic estuaries are also coherent with the weak tides along the Mediterranean coasts and lead to necessary further studies to understand the migratory behavior of glass eels in such hydro-systems.

Small Mediterranean coastal Lagoons Under Threat: Hydro-ecological Disturbances and Local Anthropogenic Pressures (Size Matters).

Mediterranean coastal lagoons are affected by multiple threats (demographic pressures, eutrophication, climate change) expected to increase in the future and impact the ecosystem services provided. Conservation norms and scientific studies usually focus on large lagoons (> 0.5 km2) due to their economic importance, while they ignore smaller lagoons. These are poorly understood and often unprotected, despite their prevalence within the Mediterranean region and their importance. Qualitative and quantitative characterisation of small lagoons, in terms of functioning and sensitivity to global and local changes, are needed to develop appropriate management strategies. For this purpose, this work provides the first inventory of all Corsican lagoons and has investigated three of them of small size (Arasu, Santa Giulia, Balistra), characterised by contrasting anthropogenic contexts (highly modified/disturbed, medium disturbance, quasi-pristine). At the regional level, 91 of the 95 lagoons identified are < 0.5 km2, making Corsica a good example for the study of small Mediterranean lagoons. The three case studies showed differences in their seasonal biogeochemical cycles and phytoplankton communities (biomass, diversity, photosynthetic efficiency). Arasu and Santa Giulia lagoons showed an increase in watershed urbanisation (+ 12% and + 6% in 30 years), high phytoplankton biomass, low diversity and blooms of potentially harmful dinoflagellates. Conversely, Balistra lagoon showed a good status overall, but some anthropogenic pollution sources within its watershed. This study demonstrates the importance of small lagoons at regional and Mediterranean scale, and provides knowledge on studied local sites but also potential applications elsewhere. The importance of an integrated approach considering lagoons within their adjacent connected systems (watershed and sea) and anthropogenic contexts is highlighted. Supplementary Information: The online version contains supplementary material available at 10.1007/s12237-023-01182-1.

Accounting for Area Sources in Air Pollution Models.

Area sources are important components of comprehensive air pollution models. The literature describes several approaches to modeling dispersion from such sources, but there is little consensus on an approach that can be applied to arbitrarily shaped area sources and is numerically efficient at the same time. This paper brings together ideas from previous work to propose an approach that meets these requirements. It is based on representing an area source as a set of line sources perpendicular to the wind direction; the number of line sources is determined by the specified precision of the concentration computed at a receptor impacted by the area source. Although AERMOD and the OML model incorporate versions of this approach, the open literature lacks an adequate description. This paper fills this important gap and also provides examples of its application. We show that different shaped area sources with the same emissions and emission density yield significantly different downwind concentration patterns. We then demonstrate the utility of the method through inverse modeling to estimate methane emissions from manure lagoons located in a dairy.

Dataset for analyzing and modelling the eutrophication processes in groundwater-coastal lagoon systems: The La Pletera lagoons case study (NE Spain).

A comprehensive hydrogeological report was conducted to determine the origin, occurrence and processes affecting nitrogen in a Mediterranean coastal aquifer-lagoon system. Water levels, hydrochemical and isotopic data was gathered during a 4-year period in the La Pletera salt marsh area (NE Spain). They were collected from the alluvial aquifer, two natural lagoons and four other permanent lagoons excavated during a restoration process (in 2002 and 2016), two watercourses (the Ter River and the Ter Vell artificial channel), 21 wells (considering six of them for groundwater sampling) and the Mediterranean Sea. Potentiometric surveys were carried out seasonally, however twelve-monthly campaigns (from November 2014 to October 2015), and nine seasonal campaigns (from January 2016 to January 2018) were conducted for hydrochemical and environmental isotopes analyses. The evolution of the water table was analysed for each well, and potentiometric maps were plotted to determine the relationship between the aquifer and the lagoons, sea, watercourses, and groundwater flow. Hydrochemical data included physicochemical data measured in situ (temperature, pH, Eh, dissolved oxygen, and electrical conductivity), major and minor ions (HCO3-, CO32-, Cl-, SO42-, F-, Br-, Ca2+, Mg2+, Na+, and K+), and nutrients (NO2-, NO3-, NH4+, Total Nitrogen (TN), PO43-, and Total Phosphorus (TP)). Environmental isotopes included stable water isotopes (δ18O and δD), nitrate (δ15NNO3 and δ18ONO3) and sulphate isotopes (δ34SSO4 and δ18OSO4). Water isotopes were analysed for all campaigns, however, nitrate and sulphate isotopes water samples were only analysed in some particular surveys (November and December 2014; January, April, June, July and August 2015). Additionally, two more surveys for sulphate isotopes were conducted in April and October of 2016. The data generated through this research may be used as a starting point to analyse the evolution of these recently restored lagoons, and their future responses to global change. In addition, this dataset may be used to model the hydrological and hydrochemical behaviour of the aquifer.